Surveillance of Viruses in <i>Varroa destructor</i> Samples Collected from Honey Bee Colonies in Ontario, Canada, between 2015 and 2019

Abstract Varroa destructor parasitism is associated with extreme honey bee ( Apis mellifera ) colony losses in the northern hemisphere. causes severe damage, including a decrease longevity and immunosuppression, acts as vector for viruses, such Deformed wing virus (DWV-A). The surveillance of viral pathogens V. samples essential to assess risks emerging virulent variants (such VDV-1) evaluate their impact on health. Thus, objective this study was identify collected Ontario, Canada, from 2015 2019 use metagenomics real time PCR (qPCR). DWV-A VDV-1 had highest abundance transcripts (7.5 log2 5.72 log2, respectively). Acute paralysis (ABPV) Bee macula were also identified. Viral identification quantification using will facilitate pathogens. This technique assist diagnostic laboratories delivering timely accurate diagnoses risk assessments, which turn help producers take adequate measures mitigate damage caused by viruses.